In achalasia cardia as we know that there is the absence of myenteric or Auerbach plexus in the muscle layer of the lower oesophagus so the increase in LES tone is mainly due to a lower concentration of acetylcholine. So, how does the BOTULINUM toxin injection decrease LES tone as we know that BOTULINUM toxin decreases the release of Acetylcholine in the neuromuscular junction?