Introduction: Escherichia coli producing extended spectrum-β-lactamases (ESBL),particularly CTX-M type ESBLs, have rapidly spread worldwide and pose a serious threat for healthcare-associated infections. We performed a molecular detection and characterization study of ESBL-related bla genes, including bla TEM, bla SHV, bla CTX-M, and blaCTX-M15, and also assessed the relationship between the phylogenetic background of strains carrying ESBL genes and the patient’s clinical outcome. Methodology: A total of 300 non-repeated, clinically significant isolates were investigated. The molecular types of ESBL genes were determined using multiplex PCR.
Phylogenetic analysis was performed using triplex PCR methods. Antibiograms and the patient’s clinical outcome were collected in a structured pro forma. Results: Among the 300 isolates, 212 (70.5%) isolates were found to carry ESBL genes. A total of…
