The recent pandemic of COVID-19 has led to the need for rapid and reliable detection of SARS-CoV-2. Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) is one such technique that can provide accurate and rapid results for the early detection of the disease. The present article focusses on the principle of working and the importance of Real-time RT-PCR for the detection of SARS-CoV-2. The real-time RT-PCR is a nuclear-derived method that has been used for the detection of specific genetic material from any pathogen, including SARS-CoV-2.
The device detects SARS-CoV-2 nucleic acids from the upper and lower respiratory specimens collected from suspected COVID-19 patients. The SARS-CoV-2 RNA is generally detectable during the acute phase of infection. Working principle of real-time RT-PCR with SARS-CoV-2 The working principle of the technique is based on nucleic acid…
