9This article focuses on the transcript level expressed in the ocular as well as non ocular tissue. Ocular tissues like retina, len epithelium and iris tissues were analysed, The non coular tissues of WBC, HeLa cell line and TM cell lines were also analyzed. Abstract PAX6 is a critical regulatory gene located on human chromosome 11p13, is expressed in many ocular tissues, and non-ocular tissues like brain, and pancreas. The expression of mRNA transcript has been investigated in this study since PAX6 is likely to play a vital role in the development of eye.
RNA was extracted from various ocular tissues including lens epithelium, retina, iris, non-ocular tissues Trabecular Meshwork cells (TM cell line), HeLa cells. Besides the mutant and wild type blood cells were also included and total RNAs were subjected to convert in to Complementary DNA (cDNA). The high sensitive reverse…
