Authors: Prutha Vaidya, Swapna Mahale, Pallavi Badade, Alisha Panjwani et al. Introduction: A wide range of barrier membranes are used in guided tissue and bone regeneration for reconstructing lost periodontium. One of the main reasons for unsuccessful regenerative procedures is the colonization and penetration of bacteria through barrier membrane into the treated site. Certain bacteria have high adherence to GTR membranes with periodontal pathogens showing greater affinity.

Antibacterial properties of amniotic fluid are well documented and demonstrated. No such factors have yet been found in amniotic membranes. The antibacterial activity if present in GTR membranes can control inflammatory lesion and collagenolytic activity of bacteria increasing the life of the membrane and treatment outcome. Study aimed to investigate the antibacterial properties of collagen and foetal barrier…