The dental pulp is unmineralised oral tissue which has soft connective tissue, lymphatic, vascular and nerve tissue in the root canal chamber and root canals of tooth in the oral cavity. The dental pulp can be regenerated with stem cells after the mechanical preparation of the infected tooth that is the hypothesis. Dental caries and periodontal infections are two major causes of infection of the dental pulp which leads to root canal preparations with a crown in most cases or extraction of the tooth itself. A root canal treatment is done by first drilling the tooth or the infected dental caries with an airotor.

Then, files like needles of different sizes are used to clean the root canals and the pulp chamber mechanically. After this the canal are irrigated with hypochlorite, hydrogen peroxide and saline, and sometimes, chlorhexidine of low concentration. Laser sterilisation can also, be…